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Critical role for p53-serine 15 phosphorylation in stimulating transactivation at p53-responsive promoters

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Allow colonies to grow overnight at 37 °C and several hours at 30 °C on selection plates containing Bluo-gal and IPTG (10 μg/ml tetracyclin, 50 μg/ml kanamycin, 7 μg/ml gentamycin, 100 μg/ml Bluo-Gal, 40 μg/ml IPTG)

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