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Subsequently, according to the method described in the previous study ( 2.7 Immunofluorescence (IF) After transcardial perfusion in mice, brain tissues were harvested and pretreated with gradient dehydration in 20% and 30% sucrose solutions at 4°C (to reduce ice crystal damage), then embedded in optimal cutting temperature compound and sectioned for 20 μm thickness using cryostat

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doi: 10.59566/IJBS.2009.5395, PMID: [DOI] [PMC free article] [PubMed] [Google Scholar] Rani I., Goyal A., Bhatnagar M., Manhas S., Goel P., Pal A., et al